Coding

Part:BBa_K1595031:Design

Designed by: Jonlin Chen   Group: iGEM15_Cornell   (2015-09-18)


EcnA and EcnB from Escherichia coli str. K-12 substr. DH10B


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 390
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Use in conjunction with strain-expressing T7 polymerase.


Source

Synthesized by DNA 2.0

References

NCBI accession number YP_001732917